Maria Luisa Mangoni

Maria Luisa Mangoni è professore associato presso il Dipartimento di Scienze Biochimiche dell’Università La Sapienza di Roma. Si laurea nel 1996 in Scienze Biologiche, presso l’Università di Roma La Sapienza dove, nel 2002, consegue il dottorato di ricerca in Biochimica. La sua attività di ricerca si concentra, a partire dal 2005, sul meccanismo molecolare che sottende l’attività antimicrobica degli AMP, mediante l’impiego di tecniche biofisiche ed esperimenti di biologia cellulare. Dal 2010 ad oggi collabora con la Prof. Alison McDermott, presso l’Università di Houston, Texas USA, per la sperimentazione di alcuni AMP in modelli animali di infezione oculare e con la dott.ssa Alessandra Bragonzi dell’Istituto San Raffaele di Milano per la sperimentazione di tali molecole in modelli di topo di fibrosi cistica.

Brevetti con FFC Ricerca

1. Use of esculentin and its derivatives for use in the treatment of cystic fibrosis
   a. Inventori: Mangoni Maria Luisa; Ferrera Loretta
   b. Titolari: Università Degli Studi Di Roma “La Sapienza”, Istituto Giannina Gaslini, FFC Ricerca.

• Luca V, Stringaro A, Colone M et al. Esculentin(1-21), an amphibian skin membrane-active peptide with potent activity on both planktonic and biofilm cells of the bacterial pathogen Pseudomonas aeruginosa. Cell Mol Life Sci. 2013 Aug;70(15):2773-86. doi: 10.1007/s00018-013-1291-7. Epub 2013 Mar 16.
• Di Grazia A, Cappiello F, Cohen H, et al. D-Amino acids incorporation in the frog skin-derived peptide esculentin-1a(1-21)NH2 is beneficial for its multiple functions. Amino Acids, 2015 Dec;47(12):2505-19. doi: 10.1007/s00726-015-2041-y. Epub 2015 Jul 11.
• Mangoni ML, Luca V, McDermott AM. Fighting microbial infections: A lesson from amphibian skin-derived esculentin-1 peptides. Peptides, 2015 Sep;71:286-95. doi: 10.1016/j.peptides.2015.04.018. Epub 2015 May 8.
• Segev-Zarko L, Saar-Dover R, Brumfeld V et al. Mechanisms of biofilm inhibition and degradation by antimicrobial peptides. Biochem J. 2015 Jun 1;468(2):259-70. doi: 10.1042/BJ20141251.
• Cappiello F, Di Grazia A, Segev-Zarko LA et al. Esculentin-1a-Derived Peptides Promote Clearance of Pseudomonas aeruginosa Internalized in Bronchial Cells of Cystic Fibrosis Patients and Lung Cell Migration: Biochemical Properties and a Plausible Mode of Action. Antimicrob Agents Chemother. 2016 Nov 21;60(12):7252-7262. Print 2016 Dec.
• Ghosh A, Bera S, Shai Y, et al. NMR structure and binding of esculentin-1a (1-21)NH2 and its diastereomer to lipopolysaccharide: Correlation with biological functions. Biochim Biophys Acta, 2016 Apr;1858(4):800-12. doi: 10.1016/j.bbamem.2015.12.027. Epub 2015 Dec 23.
• Loffredo MR, Ghosh A, Harmouche N et al.  Membrane perturbing activities and structural properties of the frog-skin derived peptide Esculentin-1a(1-21)NH2 and its Diastereomer Esc(1-21)-1c: Correlation with their antipseudomonal and cytotoxic activity. Biochim Biophys Acta. 2017 Dec;1859(12):2327-2339. doi: 10.1016/j.bbamem.2017.09.009. Epub 2017 Sep 12.
• Chen C, Mangoni ML, Di YP. In vivo therapeutic efficacy of frog skin-derived peptides against Pseudomonas aeruginosa-induced pulmonary infection. Sci Rep. 2017 Aug 17;7(1):8548. doi: 10.1038/s41598-017-08361-8.
• Casciaro B, Loffredo MR, Luca V, et al., Esculentin-1a Derived Antipseudomonal Peptides: Limited Induction of Resistance and Synergy with Aztreonam. Protein Pept Lett. 2018;25(12):1155-1162. doi: 10.2174/0929866525666181101104649.
• Cappiello F, Casciaro B, Mangoni ML. A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration. J Vis Exp. 2018 Mar 17;(133). doi: 10.3791/56825.
• Floriana Cappiello, Danilo Ranieri, Veronica Carnicelli, et al. Bronchial Epithelium Repair by Esculentin-1a-derived Antimicrobial Peptides: Involvement of metalloproteinase-9 and interleukin-8, and Evaluation of Peptides’ Immunogenicity. Sci Rep , 9 (1), 18988 2019 Dec 12
• Bruno Casciaro, Floriana Cappiello, Maria Rosa Loffredo, Francesca Ghirga, Maria Luisa Mangoni. The Potential of Frog Skin Peptides for Anti-Infective Therapies: The Case of Esculentin-1a(1-21)NH2. Curr Med Chem 2019 Jul 21 [Online ahead of print]
• Bruno Casciaro, Ivana d’Angelo, Xiaoping Zhang, et al. Poly(lactide-co-glycolide) Nanoparticles for Prolonged Therapeutic Efficacy of Esculentin-1a-Derived Antimicrobial Peptides against Pseudomonas aeruginosa Lung Infection: in Vitro and in Vivo Studies. Biomacromolecules 2019, 20, 5, 1876-1888
• Bruno Casciaro, Qiao Lin, Sergii Afonin, et al. Inhibition of Pseudomonas Aeruginosa Biofilm Formation and Expression of Virulence Genes by Selective Epimerization in the Peptide Esculentin-1a(1-21)NH 2. FEBS J, 286 (19), 3874-3891
• Maria Luisa Mangoni, Bruno Casciaro, Ivana d’Angelo, Xiaoping Zhang, Floriana Cappiello, Mariarosa Loffredo, Peter Y. Di, Francesca Ungaro. Frog skin-derived peptides for treatment of Pseudomonas aeruginosa lung infection and bronchial epithelial repair: advanced in vitro and in vivo characterization and development of polymeric nanoparticles for lung delivery. The Proceedings of the 16th Italian Convention of Investigators in Cystic Fibrosis. Multidisciplinary Respiratory Medicine, 2019, 14 (Suppl 1):5